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Exacerbation of Inflammation by Aggressive Cold Therapy: Preventing Microcellular Ice Crystal Injury. An Alternative to Conventional R.I.C.E. Therapy Stuart Tomares, M.D. Abstract Exposure to sub-0°C temperatures has long been known to cause mammalian tissue damage and cell death (frostbite). The biological mechanisms of such injury were elucidated in the 1970s and 1980s in research into the viability of cold storage …